The coomassie brilliant blue protein assay commonly known as the bradford assay 1 is widely used because of its rapid and convenient protocol as well as its relative sensitivity unfortunately there is a large degree of curvature over a broad range of protein concentrations fig. The bradford assay is linear over a short range typically from 0 µg ml to 2000 µg ml often making dilutions of a sample necessary before analysis. The linear range of these assays for bsa is 1 25 10 μg.
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